Unraveling DNA: Molecular Biology for the Laboratory |  | Authors: Michael R. Winfrey, Marc A. Rott, Alan Wortman
Publisher: Benjamin Cummings
Category: Book
List Price: $91.00
Buy Used: $27.00
as of 9/7/2010 22:40 CDT details
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New (12) Used (21) from $27.00
Seller: SBSTEXTBOOKS
Rating:  3 reviews
Sales Rank: 21,937
Media: Paperback
Pages: 400
Number Of Items: 1
Shipping Weight (lbs): 1.7
Dimensions (in): 10.9 x 8.5 x 0.7
ISBN: 0132700344
Dewey Decimal Number: 574.873282
EAN: 9780132700344
ASIN: 0132700344
Publication Date: February 1, 1997
Availability: Usually ships in 1-2 business days
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Product Description
This manual encompasses an integrated series of molecular biology laboratory exercises that involve the cloning and analysis of the bioluminescence (lux) genes from the marine bacterium Vibrio fischeri. The manual is divided into discrete units with each demonstrating one or more aspects of the cloning project. The manual is based on one of nature's most fascinating biological phenomenon: the biological production of light. This results in a recurrent theme of interest and makes the project very relevant to interdisciplinary topics such as fish symbiosis, biochemistry, biophysics, etc. Includes instruction in the basic techniques of modern molecular biology: DNA isolation and analysis, DNA restriction, agarose gel electrophoresis, ligations, transformation of recombinant DNA, preparation and screening a genomic library, restriction mapping, Southern blotting, hybridization, DNA sequencing, pulsed field gel electrophoresis. Designed for a one semester course in Molecular Biology. Also appropriate for a molecular biology component of Microbial Genetics, Genetics, Biochemistry, or Advanced Microbiology courses.
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| Customer Reviews:
 Wonderful Lab Manual February 26, 2001
Ahmed Fazly (Winona, MN United States)
This is a wonderful text clear and concise, it explores the bulk of the basic lab techniques and procedures in molecular biology. This highly readable text builds on the cumulative experiences of some highly skilled academicians and researchers. Highly recommended for any molecular biology lab course or as a useful reference.
Two thumbs up March 14, 2002
One of the best lab texts around. Provides a thorough exposure to the most commonly used molecular laboratory techniques. Highly recommended for faculty, who focus on creating a hands-on learning environment.
 This book badly needs revision March 20, 2002
Antoinette (Bethesda MD)
3 out of 3 found this review helpful
The material in the introductory text preceeding most of the exercizes lacks clarity, is poorly written and sadly confuses the reader. The introduction to the lux operon seems to be a very good example to illustrate the point. This book was made possible by a grant from the NSF and if the authors continue to market this book without revision it would be just be a travesty and an insult to intelligence. Please take note of the shortcomings of this book before decinding to buy it.
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