Culture of Animal Cells: A Manual of Basic Technique |  | Author: R. Ian Freshney
Publisher: Wiley-Liss
Category: Book
List Price: $129.95
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Seller: Sher's Warehouse
Rating:  9 reviews
Sales Rank: 70181
Media: Hardcover
Edition: 5
Pages: 696
Number Of Items: 1
Shipping Weight (lbs): 4.1
Dimensions (in): 11.1 x 8.5 x 1.6
ISBN: 0471453293
Dewey Decimal Number: 571.6381
EAN: 9780471453291
ASIN: 0471453293
Publication Date: July 29, 2005
Availability: Usually ships in 1-2 business days
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Product Description
The most complete resource on the techniques, equipment,principles, and practices of animal cell culture
Since publication of the previous edition of this benchmark text, numerous groundbreaking advances have occurred in stem cell research, cloning, tissue engineering, and in vitro toxicity testing. These and other developments have been incorporated into this fully revised and expanded Fifth Edition of Culture of Animal Cells. In addition, to answer the needs of the exponential increase in newcomers to cell culture, particularly in the biopharmaceutical industry, a completely new chapter on training in cell culture technology has been introduced.
The most complete resource on the techniques, equipment, principles, and practices of animal cell culture, this text offers a complete background related to growth of animal cells in culture. Beginning with laboratory design, safety, validation and bioethics, then continuing with preparation of media, primary culture and cell lines, through to characterization and authentication, contamination, specialized techniques, and troubleshooting, the coverage includes:
* An all-new section of training exercises, separated into basic, intermediate, and advanced procedures, cross-referenced to the relevant protocols
* New coverage of stem cells, bioethics, validation, cloning, cell signaling, in vitro toxicity testing, and tissue engineering
* An expanded full-color atlas section, with images of primary culture, cell lines, subculture, differentiation, cancer cells and transformation, three-dimensional culture, contamination, and specialized equipment
* Enhanced treatment of troubleshooting, with full cross-referencing to the relevant protocols and sections of text
* Fully updated references
* The clearest, most consistent presentation of step-by-step protocols available
* Numerous diagrams, photographs, tables, and charts
* Detailed and up-to-date information on reagent preparation and sourcing of materials and equipment, including a fully updated list of suppliers and other resources with Web sites
Indispensable for clinical and biopharmaceutical researchers and scientists, students, trainees, and technicians, this landmark text presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells.
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| Customer Reviews:
Showing reviews 1-5 of 9
A SUPERB Manual of Basic Cell CultureTechnique September 12, 2001
P. Barriga (Montreal Quebec Canada)
19 out of 20 found this review helpful
I purchased this book a year ago to use in a clinical virology laboratory. Although many of the recipes used in my field are essentially set, this book allowed for broad learning in the skill of cell culture. I learned many principles of cell culture that were poorly explained or not mentioned in clinical literature and books.This book is well laid out, the chapters well written, and the information is comprehensive. Subjects include (besides the usual stuff of cell culture) chapters covering equipment materials, safety, contamination, scale up, a reagent appendix, and supplier's listing. This book is an excellent choice for the serious beginner. The expert will certainly find this book of value because even experts are only expert at what they do routinely and there is so much to know. This would be a great training source for research or clinical laboratories who perform cell culture.
The best book on cell culture January 8, 2005
Mike_brazil (Rockville, MD)
9 out of 9 found this review helpful
Freshney's Culture of Animal Cells is a great book. It is writen on an easy style, it is concise and complete at the same time and it does teach you all the basics (and a little bit more) of cell culture. When I was doing my masters I didn't have a lot of help with my cell culture experiments and this book was fundamental for my research. The book covers everything, starting from the biology of cultured cells, equipments, design of the lab and asseptic technique and going all the way to primary culture, cloning and selection, cell separation, characterization, differentiation and more. The book (hard cover) is pretty resistant and it easily stands going to the bench (where you surely will be using it a lot). If you are thinking about spending money on a book about cell culture techniques this is the book! Don't think twice.
Best Basic Book on Tisssue Culture July 7, 2006
Constance Oliver (Ribeirão Preto, SP, Brazil)
1 out of 1 found this review helpful
For many years Freshney's Culture of Animal Cells has been the gold standard of tissue culture reference books. This new edition adds and updates many techniques and continues to be the best book in its area. I use it extensively both for my own research and in teaching classes on tissue culture.
Still excellent at 3 years old... June 29, 2004
Even though this book was published in 2001, it is still an irreplaceable source of info in my lab. The pictures and tables are wonderful. The only drawback is that is lacks info on a few cell lines (THP1, for example).
useful book August 9, 2007
Dolly Lemon (San Francisco, CA)
There are not a lot of glossy pretty pictures, which is a good thing for a no-nonsense instruction manual that gets the point across with what pictures it did have. I found it to be a very good complement to my cell culture class. Everything I learned in the lab was put into context nicely.
Showing reviews 1-5 of 9
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